VII. AUTOCLAVES  print this page
An autoclave is readily available.		If NO, then		An autoclave must be readily available. Contact your building administrator to locate the nearest autoclave.
Quality control and quality assurance testing is performed on a regular basis.		If NO, then		Autoclaves must be tested for quality assurance. Use of a biological indicator (such as Bacillus stearothermophilus spore strips) or a chemical indicator that measures both time and temperature (such as Sterigage) is the most reliable way to check for autoclave effectiveness. Bury the indicator in the center of the load to validate adequate steam penetration. Keep a log book to record the results.
Area around the autoclave is clean and free of clutter.		If NO, then		Remove anything that does not belong in the area and ensure that the space is cleaned on a regular basis.
Localized ventilation is associated with the autoclave and operational.		If NO, then		Contact EHRS if the autoclave does not have an exhaust canopy over the door of the autoclave or if it is not operational.
Do you use methods of sterilization other than autoclaving (ethylene oxide, formaldehyde, hydrogen peroxide, etc.)?		If YES, then		Additional training may be required. Contact EHRS for recommendations.
Secondary containment is used when autoclaving infectious waste bags, sharps containers, reusable laboratory glassware, etc.		If NO, then		Place all items to be autoclaved (infectious waste bags, sharps containers, reusable laboratory glassware, etc.) into a secondary container (ie., Nalgene pan) to ensure personal safety (ie., burns) and damage to the autoclave (clogged effluent drain, coagulated plastic on autoclave racks, etc.).
An autoclave rack is present and used during each cycle.		If NO, then		Never place items directly on the floor of the autoclave chamber. Ensure that an autoclave rack is present and used during each cycle.
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